July 1, 2017

Quality in brewing

Quality in brewing


  • means: consistently meet the specification
  • specification: which beer style? how to measure them? 
  • consistency: depends on scientific facts and measurements
  • homogeneity: 2 row barley is more homogeneous than 6 row barley, easy to meet the quality standard
  • basic rules of quality control: set-compare-fix
  • establishment of specification: via human sense or instrumental analysis
  • spec in/out

Quality control via sampling


  1. raw material: each delivery
  2. brewhouse operation: each batch
  3. unfermented wort: each batch
  4. yeast: each batch, every pitch
  5. fermenting beer: every 12 hours
  6. fermented beer and beer ready for filtration: each tank
  7. packaging material: each delivery
  8. beer in package: each code
  9. plant: number of tanks per week
  10. cleaning material: specific sample of material per week

Parameters in specification


  1. ABV: alcohol
  2. OG, FG: body, richness in flavor
  3. pH: yeast performance, hop utilization, and bitterness, microorganism growth
  4. beer color
  5. IBU
  6. flavor compounds
  7. DO2: origin of oxidation 
  8. CO2: over carbonation? 
  9. N2: head retention
  10. beer flavor: match for style? 
  11. clarity
  12. head stability
  13. sterility: wild yeast or bacteria

Main components of contamination


  • chemical
  • microbiological
  • physical

Anaerobic organism


  • obligate anaerobe: cannot live with oxygen
  • aero-tolerant organism: no need oxygen to live but can tolerate
  • facultative anaerobe: no need oxygen to live but can use, brewer's yeast

Microorganism

in unpitched wort: 


  • obesumbacteria(Hafnia), grow with yeast and found in top fermentation
  • escherichia(e-coli), originated from contaminated water
  • enterobacter, cannot grow in pH 4.3 and vigorously grow in wort(not beer). produce DMS, diacetyl off flavor

in fermentation: 


  • pediococcus, produce lactic acid and diacetyl
  • lactobacillus, produce lactic acid and diacetyl
  • wild yeast, produce biofilm, super attenuation

wild yeast: 


  • pichia, vigorously grow in the air and produce biofilm
  • candida, vigorously grow in the air and produce biofilm
  • saccharomyces diastaticus, super attenuation
  • torulopsis, produce precipitate, cause haze
  • brettanomyces, long slow growth and give sourness, off flavor to beer
  • hansenula, vigorously grow in the air and produce biofilm

Control over microorganism


  • equipment design for easy cleaning
  • efficient cleaning and sterilization
  • sterilizing product and raw material: boiling, filtering, pasteurization
  • environment against microorganism growth: low temperature(1'c), lower pH(3.5~4.5), washing yeast, pasteurization, fresh yeast

Sensory evaluation

4-vinyl guaiacol(phenolic)


  • how to: 맥주 잔을 손으로 덮어 향이 맥주잔 내 가득 발산하게 한 뒤 냄새를 맡아봄.
  • mainly considered as off flavor, but one of the profiles in wheat beer
  • word: clove like, spicy, herbal, 치과냄새. 치과 치료 받고 우루룩하는 느낌
  • origin: wild yeast, bacteria infection
  • threshold: 0.2mg/L
  • similar to Isobutyraldehyde, Sulphur dioxdie, Vanillin

Diacetyl


  • how to: 유리잔 채로 맥주를 흔들어 향 발산시켜 냄새를 맡아봄. 인지 어려울 경우 입 안에 머금어 미끈거리는 느낌 확인
  • off flavor for pale lager, not bad for ale and stout
  • word: buttery, butterscotch, honey, caramel, 둥글고 들큰한 향
  • origin: alpha-acetolactate, bacteria infection, lactobacillus, pediococcus
  • threshold: 10-40ug/L(2,3-butanedione)
  • similar to Butyric acid, Vanillin, Isobutyraldehyde

Citric acid(sour)


  • how to: 입 안에 소량의 맥주를 머금고 혀를 몇 초간 굴린 후 삼킴
  • when too much, considered as off flavor
  • word: acidic, lemon, sour milk, 신맛. 레몬즙 맛. 피니시까지 계속 시기만 함
  • origin: wrong fermentation environment, bacteria infection
  • threshold: 60-170mg/L
  • similar to Acetic acid, Lactic acid, Astringent

H2S(Hydrogen sulphide)


  • how to: 향이 발산되게 흔들어 냄새를 맡는다
  • bring freshness with low amounts, off flavor with high amounts
  • word: rotten eggs, sulphidic, 썩은 달걀
  • origin: by product of wrong fermentation, bacteria infection
  • threshold: 4ug/L
  • similar to Sulphur dioxide, Ethanethiol, Methanethiol

Oxidation(Trans-2-nonenal, T2N, Parpery)


  • how to: 유리잔 채로 맥주를 흔들어 향 발산시켜 냄새를 맡아봄.
  • mainly found in aging beer, produced during production and distribution, favored in high temperature
  • word: cardboard, oxidized, papery, 오래된 맥주, 마분지, 골판지
  • origin: oxidation
  • threshold: 50-100ng/L
  • similar to 2,4,6-Trichloroanisole, 2-Ethyl fenchol, Isobutyraldehyde

Lightstruck(3-methyl-2-butane-1-thiol, 3MBT)


  • how to: 팔 길이 안에서 냄새를 맡는다
  • exposed by sunlight
  • word: skunky, sunstruck, 음식쓰레기 냄새, 구린내
  • origin: distortion of the chemical structure of iso-alpha acid
  • threshold: 4-30ng/L(3-Methyl-3-butane-1-thiol)
  • similar to sulphur dioxide, cis-3-Hexenol, Ethanethiol

Dimethyl sulphide(DMS)


  • how to: 맥주를 흔들어 향 발산시켜 냄새를 맡아봄.
  • sweet corn flavor, good for certain pale lager style
  • word: sweet corn, cooked vegetable, 들큰한 스위트콘, 스위트콘 통조림
  • origin: imperfect malt modification(kiln), bacteria infection, S-methyl methionine and dimethyl sulphoxide
  • threshold: 25ug/L
  • similar to Methyl thioacetate, Ethanethiol, Dimethyl trisulphide

Acetaldehyde


  • exist in every beer with low content, but off flavor with high content
  • word: aldehyde-like, green apple, oil painting, 사과주 냄새
  • origin: short fermentation, dissolved oxygen, pediococcus
  • threshold: 5-15mg/L

Metalic


  • how to: 입 안에 소량의 맥주를 머금고 혀를 몇 초간 굴린 후 삼킴. 손등에 맥주를 비벼 금속 향이 나는지 확인
  • contact with metal pipe or machine, can, bottle cap or oxidation of lipids
  • word: inky, blood-like, tinny, 피맛, 뒷맛이 금속맛, 메아리처럼 퍼짐
  • origin: raw material, contact with metal, diatomite
  • threshold: 1~2.7mg/L
  • similar to Sulphitic, Citric acid, T2N

Ethylmercaptan/Ethanethiaol(Mercaptan/Methanethiol)


  • how to: 유리잔 채로 맥주를 흔들어 향 발산시켜 냄새를 맡아봄. 
  • rotten gas, sulphur, burnt match
  • word: drain, rotten vegetable, neutral gas, 하수관 냄새, 오래된 배관의 퀘퀘함
  • origin: produced during conditioning, yeast autolysis
  • threshold: 1ug/L
  • similar to Ethanethiol, Dimathyl trisulphide, Sulphur dioxide

Butyric


  • how to: 맥주 잔을 손으로 덮어 향이 발산하게 흔들어줌. 향이 묻은 손의 냄새를 맡으며 인지할 때까지 반복
  • rotten flavor, baby vomit
  • word: rancid, babysic, putrid, 아기 토 냄새
  • origin: bacteria(pectinatus, megasphaera) infection during wort production or packaging
  • threshold: 2-3mg/L
  • similar to Isovaleric acid, Ethyl butyrate, 2,3-Butanedione

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